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. 2000 Mar 6;148(5):1047–1062. doi: 10.1083/jcb.148.5.1047

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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SdpII colocalizes with dynamin in NGF-stimulated PC12 cells. PC12 cells, differentiated with 50 μg/ml NGF for 2 d, were fixed and double-labeled with antibodies directed against SdpII (a, d, e, and j), dynamin (b, f, and g), or transferrin receptor (k). SdpII localizes to discrete areas in cortical cell regions (a), in growth cones (e), and in the varicosities of processes (d) that are marked by the endocytic machinery of the cells, as indicated by staining for dynamin (b, f, and g). Colocalization of SdpII with transferrin receptor is partial and most prominent at the cell surface (l). Bars: (a–c, j–l) 5 μm; (d–i) 3 μm.