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. 2000 Mar 6;148(5):945–956. doi: 10.1083/jcb.148.5.945

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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We compared lipid droplet distribution in embryos laid by wild-type flies (Oregon-R) or by flies transheterozygous for two weak alleles of Dhc64C, the gene for the heavy chain of cytoplasmic dynein (Gepner et al. 1996). Fixed embryos were stained for lipid droplets as described in Welte et al. 1998. In both genotypes, droplets accumulate in the center, towards the plus end of microtubules, in early cycle 14 (not shown); in Dhc64C mutants, they fail to redistribute towards the minus ends (the periphery) during gastrulation. Bar, 200 μm.