Figure 6.

Distribution of lengths of droplet travel in the minus end direction (35-nm bins). The location of individual droplets as a function of time was determined with nanometer-level resolution using centroid analysis (Welte et al. 1998). Histograms shown are of run distances of droplets moving in the minus end direction during phase II for wild-type (A) and Dhc64C mutant (B) embryos, and during phase III in wild-type embryos (C). Very short runs were difficult to detect, so many such runs (first bin) were presumably missed. In both cases, the general shape of the histograms was the same and well fit by the sum of two decaying exponentials (solid lines, see also Table ). The exponential fit started with bin 2, and used all bins with five or more counts.