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Immunofluores- cence staining with A2B5, O1, and anti-nestin antibodies of adult OPCs freshly isolated from P50 and P300 rat optic nerve. The cells were cultured without TH in slide flasks in PDGF (P50 cells) or PDGF plus NRG (P300 cells) for 2 d before they were stained. In C, cell nuclei were stained with propidium iodide (red). Bar, 10 μm.
