Figure 4.

Random cell migration is reduced in cells expressing the F31/118 mutations on paxillin. Stable transfectants were allowed to attach on plates coated with 20 μg/ml of collagen for 2 h. Cell motility was evaluated by tracking individual cells for 12 h with a Leica inverted microscope connected to a computer using the Metamorph software. (A) Values obtained from measuring the tracks of cells are presented as micrometers per hour. Each bar represents the mean of at least three independent experiments ± SD. (B) Representative migration tracks formed by clones C1 (a), P1 and P2 (b and c), F31-1 and F31-3 (e and f), F118-1 (d), and Fx2 and Fx3 (g and h).