Figure 6.

Histology and immunohistochemistry of longitudinal spleen sections from week three wild-type (control) and collagen X KO mice with perinatal lethality (KO MUT). A, Altered splenic architecture in the KO mutant is revealed by H&E as poorly distinguishable lymphatic nodules and red pulp reduction, by MOMA staining for splenic macrophages surrounding periarteriolar sheaths as decreased and disorderly nodules with decreased internodular distance, and by TER 119 staining of erythroid cells as a depleted red pulp. B, Immunohistochemical localization of T and B lymphocytes reveals in the KO mutant: unaltered distribution of CD4⁺ and CD8⁺ T cells, but a reduction in nodular size and internodular space, and more diffuse B220 staining for B cells. Bars, 100 μm.