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. 2000 May 15;149(4):889–900. doi: 10.1083/jcb.149.4.889

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Morphology of GFP-Nter-TIVAMP–expressing cells. PC12 cells transfected with GFP-Nter-TIVAMP and treated with staurosporine as in Fig. 5 were fixed, processed for double fluorescence by combining direct GFP fluorescence detection with indirect immunofluorescence detection using the indicated antibodies. Representative GFP-Nter-TIVAMP–transfected cells without or with short neurite(s) are shown in horizontal confocal sections. Syntaxin (Stx) 1 and 6 and synaptobrevin 2 (Sb2) have a localization similar in untransfected as in GFP-Nter-TIVAMP–expressing cells. Synaptotagmin I immunoreactivity was weaker in GFP-Nter-TIVAMP–transfected cells than in untransfected cells. Bar, 10 μm.