Table 1.
Nuclear Migration and Microtubule–Cortex Interactions during G1
| Strain | Time nucleus is positionedat neck relative to bud emergence | % Nuclear movements associatedwith microtubule shrinkage at budcortex or incipient bud site | Frequency of microtubulecapture/shrinkage | Frequency of microtubulegrowth and pushing |
|---|---|---|---|---|
| min | min−1 | min−1 | ||
| Wild-type | 0.8 ± 3.0 before (19) | 70.4 ± 6.2 (54) | 0.126 (13) | 0.078 (8) |
| yeb1Δ | 36.7 ± 4.6 after (16) | 42.6 ± 6.3 (61) | 0.066 (6) | 0.036 (3) |
| ts-arp1 | 1.8 ± 1.1 before (122) | 57.4 ± 5.8 (62) | 0.036 (3) | 0.060 (5) |
| ts-arp1 yeb1Δ | 15.1 ± 5.3 after (33) | 58.1 ± 8.9 (31) | 0.048 (3) | 0.048 (3) |
Data for the first two columns were obtained from time-lapse movies (1-min intervals). Data for the last two columns were obtained from real-time movies (5 fps). Values for times are mean ± SEM. Values for percentages are mean ± SEP. For times and percentages, numbers of cells are in parentheses. For frequencies, numbers of events are in parentheses. Frequencies are of microtubule shrinkage or growth, associated with nuclear movements, and were obtained from 27 wild-type cells, 22 yeb1Δ cells, 21 ts-arp1 cells, and 16 ts-arp1 yeb1Δ cells. Numbers in bold type are significantly different from wild-type values, with P < 0.01.