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. 2000 May 15;149(4):767–774. doi: 10.1083/jcb.149.4.767

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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(a) Vitreous ice–embedded microtubules observed in interphasic extracts supplemented with isolated centrosomes and incubated for 20 s at 25°C. E, extensions observed at microtubule ends; and C, centriole. (b and c) Vitreous ice–embedded microtubules observed in interphasic extracts in the presence of 7.5 μM recombinant Op18/stathmin. Addition of Op18/stathmin in this condition induces the disappearance of sheets at microtubule ends (black arrows). F, frayed end; and B, blunt end. Bar, 200 nm.

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