Figure 5.

Visualization of Rab proteins on endosomal membrane domains by time-lapse video microscopy. (A) Time-lapse series of A431 cells coexpressing CFP and YFP fusion proteins of Rab4, Rab5, and Rab11. (B) Differential response of Rab4, Rab5, and Rab11 membrane domains to treatment with BFA. Single frames from time-lapse series (compare videos 4–6). (Top) Cells expressing GFP-Rab4 after internalization of rhodamine transferrin for 10 min followed by treatment with 5 μg/ml BFA for 10 min. (Middle and bottom) Cells expressing combinations of Rab4 and Rab11, or Rab4 and Rab5. Bars: (A) 1 μm; (B) 1 μm.