Table 2.

Quantitation of Rab4, Rab5, and Rab11 Overlap on the Same Endosomes

	Rab5 only	Rab4 and 5	Rab5 and 11	Rab4, 5, and 11
Rab 5 endosomes	48 ± 5	43 ± 8	23 ± 2	15 ± 5
	Rab4 only	Rab4 and 5	Rab4 and 11	Rab4, 5, and 11
Rab 4 endosomes, steady state/2 min + 3 min chase	24 ± 4/ND	52 ± 6/55 ± 1	50 ± 1/ND	23 ± 1/ND
Rab4 endosomes 50 nM wortmannin	ND	65.5 ± 4	34.5 ± 9	ND

Fluorescence analysis: Cells coexpressing YFP-Rab4 and CFP-Rab5 were stained with antibodies against Rab11. Numbers represent the percentage of endosomes labeled for the reference Rab protein only, or combinations of two or three Rab proteins.