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. 2000 May 15;149(4):825–834. doi: 10.1083/jcb.149.4.825

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Localization of MyoD and myosin mRNAs in the somites and segmental plate mesoderm of the stage 14 embryo. Photomicrographs in A, E, and I are DIC images of the merged images of bis-benzamide-labeled nuclei and Cy3-labeled dendrimers in B, F, and J, respectively. MyoD dendrimers were concentrated in the dorsal–medial portion of the dermomyotome (dm; B). A few cells of the sclerotome (sc) and neural tube (nt) also were labeled. The pattern of labeling with myosin dendrimers was similar to, but less abundant than, MyoD (D). GAPDH dendrimers produced intense fluorescence throughout the dermomyotome and sclerotome (G), whereas dendrimers lacking a specific recognition sequence did not bind to the somite (C). A subpopulation of cells in the epithelial somite (s; F) and segmental plate (sp; J) contained MyoD dendrimers. A few myosin dendrimers were found in the segmental plate (H). Bar, 9 μm.