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. 2000 May 15;149(4):825–834. doi: 10.1083/jcb.149.4.825

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Localization of MyoD in pregastrulating embryos. The areas outlined in the drawing of the pregastrulating embryo (A) are shown in B–H. Photomicrographs in B and E are the DIC images of the merged images of bis-benzamide-labeled nuclei and Cy3-labeled dendrimers in C and F, respectively. The stage X embryo contained ∼20 MyoD positive cells in the posterior epiblast (C). MyoD fluorescence extended more laterally in the posterior epiblast in the stage XII embryo (F). By stage 2, MyoD positive cells also were present in the lateral epiblast (H). Myosin dendrimers did not label stages X (D) or XII (G) embryos. Bar, 15 μm.