Table 1.
Activation of PvanH and PvanRa by VanRa
| Regulator, kinase | lacZ fusion* | β-Galactosidase Sp
Act
|
|
|---|---|---|---|
| −Rha | +Rha | ||
| None | PvanH | 0.7 ± 0.1 | N.D. |
| VanRa | PvanH | 0.9 ± 0.3 | N.D. |
| VanRa, PrhaB-vanSa | PvanH | 0.8 ± 0.1 | 31.2 ± 4.1 |
| None | PvanRa | 12.8 ± 0.9 | N.D. |
| VanRa | PvanRa | 14.7 ± 1.7 | N.D. |
| VanRa, PrhaB-vanSa | PvanRa | 10.4 ± 1.7 | 46.7 ± 4.2 |
Cells were assayed after 16–20 hr growth in 0.10% glycerol-Mops-2 mM Pi medium without (−Rha) or with (+Rha) rhamnose. Specific activity (Sp Act) units are nanomoles of product formed per cell optical density at 420 nm. Means of three or more determinations with SD are given. N.D., not determined.
*
Strains are integrants of BW24381 with single copies of the plasmids in parenthesis: JCS182 (pJS94), JCS184 (pJS96), JCS196 (pJS94, pSLF55), JCS207 (pJS96, pSLF55), JCS230 (pJS94, pSLF55, pJS87), and JCS345 (pJS96, pSLF55, pJS87).