Table 2.
Activation of PvanRb and PvanY by VanRb
| Regulator, kinase | lacZ fusion* | Carbon source | β-Galactosidase Sp Act
|
|
|---|---|---|---|---|
| −Rha | +Rha | |||
| None | PvanY | Fru | 7.6 ± 1.2 | N.D. |
| VanRb | PvanY | Fru | 42.5 ± 3.8 | N.D. |
| VanRSb | PvanY | Fru | 2.6 ± 0.5 | N.D. |
| None | PvanRb | Fru | 8.5 ± 0.9 | N.D. |
| VanRb | PvanRb | Fru | 40.8 ± 0.5 | N.D. |
| VanRSb | PvanRb | Fru | 2.3 ± 0.4 | N.D. |
| VanRb, PrhaB-vanSb | PvanY | Gly | 59.2 ± 3.0 | 6.7 ± 0.8 |
| VanRb, PrhaB-vanSb | PvanY | Fru | 44.7 ± 2.6 | 12.5 ± 1.3 |
| VanRb, PrhaB-vanSb | PvanY | Glu | 47.9 ± 0.4 | 40.5 ± 2.2 |
| VanRa | PvanY | Fru | 8.7 ± 1.3 | N.D. |
| VanRa, PrhaB-vanSa | PvanY | Fru | 7.4 ± 0.8 | 7.6 ± 1.0 |
| VanRa | PvanRb | Fru | 11.0 ± 1.4 | N.D. |
| VanRa, PrhaB-vanSa | PvanRb | Fru | 8.4 ± 0.9 | 8.7 ± 0.7 |
| VanRb | PvanH | Fru | 0.9 ± 0.5 | N.D. |
| VanRb | PvanRa | Fru | 13.6 ± 3.5 | N.D. |
Cells were assayed after growth in 0.06% glucose (Glu)-, 0.10% glycerol (Gly)-, or 0.06% fructose (Fru)-Mops-2 mM Pi medium as in Table 1. N.D., not determined.
*
Strains are integrants of BW24381: JCS186 (pJS97), JCS188 (pJS98), JCS197 (pJS97, pSLF55), JCS198 (pJS98, pSLF55), JCS199 (pJS94, pJS107), JCS200 (pJS96, pJS107), JCS201 (pJS97, pJS107), JCS202 (pJS98, pJS107), JCS237 (pJS97, pJS107, pJS102), JCS240 (pJS97, pJS109), JCS241 (pJS98, pJS109), JCS347 (pJS97, pSLF55, pJS87), and JCS349 (pJS98, pSLF55, pJS87).