Table 3.
Activation of VanRa and VanRb by VanSa, VanSb, and PhoR
| Regulator, kinase | lacZ fusion* | Carbon source | β-Galactosidase Sp Act
|
|
|---|---|---|---|---|
| −Rha | +Rha | |||
| None | PvanH | Fru | 0.7 ± 0.1 | N.D. |
| VanRa | PvanH | Fru | 0.9 ± 0.3 | N.D. |
| VanRa, PrhaB-vanSa | PvanH | Fru | 0.7 ± 0.0 | 15.2 ± 1.9 |
| VanRa, PrhaB-vanSb | PvanH | Fru | 0.8 ± 0.1 | 0.8 ± 0.1 |
| VanRa, PrhaB-vanSb | PvanH | Gly | 0.7 ± 0.1 | 0.7 ± 0.1 |
| VanRa, PrhaB-phoR | PvanH | Fru | 0.7 ± 0.2 | 21.2 ± 1.3 |
| VanRa, PrhaB-phoR | PvanH | Gly | 0.8 ± 0.2 | 47.6 ± 8.4 |
| None | PvanY | Fru | 7.6 ± 1.2 | N.D. |
| VanRb | PvanY | Fru | 42.5 ± 3.8 | N.D. |
| VanRb, PrhaB-vanSa | PvanY | Fru | 38.7 ± 2.4 | 43.1 ± 1.8 |
| VanRb, PrhaB-vanSa | PvanY | Gly | 22.7 ± 2.7 | 37.4 ± 3.3 |
| VanRb, PrhaB-phoR | PvanY | Fru | 32.2 ± 2.3 | 34.2 ± 1.1 |
| VanRb, PrhaB-phoR | PvanY | Gly | 26.0 ± 5.2 | 37.0 ± 2.6 |
Cells were assayed as in Table 2. N.D., not determined.
*
Strains are integrants of BW24381; JCS182 (pJS94), JCS186 (pJS97), JCS196 (pJS94, pSLF55), JCS201 (pJS97, pJS107), JCS229 (pJS94, pSLF55, pAH151), JCS230 (pJS94, pSLF55, pJS87), JCS231 (pJS94, pSLF55, pJS102), JCS238 (pJS97, pJS107, pJS87), and JCS239 (pJS97, pJS107, pAH151).