Figure 2. Genes implicated in diaphragm morphogenesis are expressed concomitantly in mesenchymal cells of the lungs and heart.

Adjacent tissue sections from E11.5 wild-type mice were subjected to immunoperioxidase staining with antibodies against FOG-2 (A) or GATA-4 (B) and then counterstained with toluidine blue. Note that FOG-2 and GATA-4 are co-expressed in the central tendon of the diaphragm, lung mesenchyme, and the heart. Abbreviations: ct, central tendon; ht, heart; lm, lung mesenchyme. Bars, 75 μm.