Figure 1.

Characterization of Wild-Type RCA (RCA1) and Thermostable Variants (183H12, 301C7, and 382D8).

(A) Rubisco activity after activation by activase after treatment at 25°C (white), 40°C (gray), and 45°C (black). Activase proteins were incubated at the indicated temperatures for 15 min prior to assaying at 25°C.

(B) Activation of Rubisco under catalytic conditions at 25°C (white) and 40°C (gray).

(C) ATPase activity (relative to RCA1 activity at 25°C) of activase proteins incubated at the indicated temperatures for 15 min prior to assaying at 25°C.