Characterization of the Δrca Mutant at Ambient CO2.
(A) Immunoblot analysis from leaves of Arabidopsis wild-type (RCA/RCA), heterozygous (RCA/Δrca), and homozygous (Δrca/Δrca) plants. The blot was immunodecorated with polyclonal antibodies raised against the recombinant Arabidopsis RCA1.
(B) Photosynthetic performance (Fq′/Fm′) of 3-week old wild-type (top) and Δrca (bottom) plants as measured using fluorescence image analysis.
(C) Leaf area (mm2) of the plants described in (B) (50 plants per phenotype) at the indicted age.
(D) Eight-week-old wild-type (top) and Δrca (bottom) plants.