Figure 6.

Ectopic Expression of CYCB1;1 in the mid Mutants and Rescue by CYCB1;2.

(A) Ectopic expression of CYCB1;1:GUS in mid mutant trichomes. Arrowheads indicate GUS-stained trichomes. Inset is an enlargement of a trichome on the same leaf.

(B) Ectopic expression of CYCB1;1:GUS in the mid elongated hypocotyl cell.

(C) to (H) Rescue of the trichome cell size and DNA endoreduplication defects by overexpression of the mitotic cyclin B1;2 in the mid mutant trichomes. Bar in (C) = 200 μm for (C) to (E) and 50 μm for (F) to (H).

(C) mid-2 trichome.

(D) Wild-type trichome.

(E) mid-2 GL2:CYCB1;2 trichome.

(F) to (H) DAPI-stained nuclei in trichomes.

(F) mid-2.

(G) The wild type.

(H) mid-2 GL2:CYCB1;2. Note restored nuclear size and brightly stained chromocenters in.