Figure 10.

Pcl12 Is Required for Teliospore Germination.

(A) Images of control and Δpcl12 teliospores germinated on CM glucose–containing agar slides after 1 d of incubation. Note that wild-type teliospores extend a promycelium, from which haploid progeny are generated. In mutant teliospores, no germination is observed and, scarcely, a short and thick filament is observed in some teliospores (inset). Bars = 20 μm. Inset bar = 5 μm.

(B) Quantification of teliospore germination after 12 and 36 h. Error bars represent values ± sd.

(C) Quantitative analysis of tumor formation in plants sprayed with wild-type and Δpcl12 teliospores and incubated for 14 d after infection. Error bars represent values ± sd.