Figure 3.

Abundance of Pn FT1 and Pn FT2 Transcripts in Pharbitis cv Violet Shows a Circadian Rhythm, and Their Expression Requires Darkness.

Quantification of Pn FT1 and Pn FT2 from RT-PCR followed by DNA gel blotting. Plants were grown under LDs or SDs for 3 d and then shifted to continuous light (LL) or continuous darkness (DD). Cotyledons were harvested for RNA extraction every 4 h during the last LD or SD and every 3 h for 48 h in LL and DD. Solid black line indicates plants transferred from SD to DD. Dotted black line indicates plants transferred from SD to LL. Solid blue line indicates plants transferred from LD to DD. Dotted blue line indicates plants transferred from LD to LL. Error bars show se of three PCR experiments. A biological replicate provided a similar result.