Figure 3.

The DEK1 Calpain Cys Proteinase Domain Alone Is Unable to Fully Complement the dek1 Mutant Phenotype in Arabidopsis.

(A) Arabidopsis seeds from a DEK1/dek1-1 plant transformed with the At DEK1-CALP expression cassette under the control of the At DEK1 promoter, showing segregation of a wild-type seed (WT), an intermediate phenotype seed (dek1-int), and a collapsed dek1-1 seed (dek1).

(B) to (D) Longitudinal sections of Arabidopsis seed types shown in (A): wild-type seed (B); intermediate seed type (C); and typical collapsed dek1-1 seed (D).

(E) Detail from a wild-type seed with a peripheral aleurone layer (arrow).

(F) Detail from an intermediate seed type lacking aleurone differentiation in the peripheral position of the endosperm (arrow).

(G) An embryo from intermediate type seeds.

(H) A typical dek1-1 embryo.

E, embryo; EN, endosperm; SC, seed coat. Bars = 50 μm.