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. 2007 Oct;19(10):3212–3229. doi: 10.1105/tpc.107.054155

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Copyright © 2007, American Society of Plant Biologists

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Figure 4. — Formation of Methylcinnamate in Nondesalted Crude Protein Extracts of MC Glandular Trichomes.

Assay products were analyzed by GC-MS. [U-¹³C]Cinnamate was provided as substrate in all assays; other assay components are as indicated in the graphs. [U-¹³C]Methylcinnamate was formed only when AdoMet (SAM) and plant protein extracts were added (A). Endogenous methylcinnamate from the crude protein extracts is present in all samples ([A] to [C]) except for the no-protein control (D). In (E), fragment ions observed in the MS spectra are readily explained and support the identity of the product as [U-¹³C]methylcinnamate (compared with ions from natural abundance methylcinnamate).