Table 1.
Substitution Pattern
|
Relative Specific Activity
|
||||||||
---|---|---|---|---|---|---|---|---|---|
Substrate | Structure | R1 | R2 | R3 | R4 | CCMT1 | CCMT2 | CCMT3 | CCMT1-H160M |
CA-like | |||||||||
trans-Cinnamic acid (CA) | H | H | H | H | 100 (1.7) | 100 (7.1) | 100 (15.1) | 100 (7.5) | |
Hydrocinnamic acida | H | H | H | H | 21.4 (1.0) | 6.8 (3.4) | 39.3 (5.5) | 21.8 (1.9) | |
p-Coumaric acid (pCA) | H | H | OH | H | 29.6 (3.9) | 24.5 (5.7) | 63.5 (8.0) | 80.2 (1.6) | |
4-Hydroxyhydrocinnamic acida | H | H | OH | H | 22.4 (4.8) | 19.1 (6.5) | 23.5 (2.0) | 0.9 (0.7) | |
m-Coumaric acid | H | OH | H | H | 6.8 (1.5) | 0 | 0 | 0 | |
o-Coumaric acid | OH | H | H | H | 0 | 0 | 0 | 0 | |
Caffeic acid | H | OH | OH | H | 0 | 0 | 0 | 0 | |
Hydrocaffeic acida | H | OH | OH | H | 0 | 0 | 0 | 0 | |
Ferulic acid | H | OMe | OH | H | 0 | 0 | 0 | 0 | |
Hydroferulic acida | H | OMe | OH | H | 0 | 0 | 0 | 0 | |
3,4-Dimethoxycinnamic acid | H | OMe | OMe | H | 0 | 0 | 0 | 0 | |
Sinapic acid | H | OMe | OH | OMe | 0 | 0 | 0 | 0 | |
BA-like | |||||||||
Benzoic acid (BA) | H | H | H | H | 7.8 (0.8) | 10.1 (4.8) | 6.2 (1.3) | 161.9 (2.2) | |
Salicylic acid (SA) | OH | H | H | H | 0 | 0 | 0 | 61.1 (0.4) | |
Anthranilic acid | NH2 | H | H | H | 0 | 0 | 0 | 20.5 (0.7) | |
Shikimic acid | H | OH | OH | OH | 0 | 0 | 0 | 0 | |
Other | |||||||||
Phenylacetic acid | 0 | 0 | 0 | 0 | |||||
Phenylpyruvic acid | 0 | 0 | 0 | 0 | |||||
Indole-3-acetic acid | 0 | 0 | 0 | 0 | |||||
Jasmonic acid | 0 | 0 | 0 | 0 |
These substrates have a single carbon bond between C7 and C8 and not the double bond shown in the generalized structures in the table.