Table 2.
Comparison of Km, Kcat, and Kcat/Km Values for Basil CCMT Isoforms and CCMT1-H160M
| Substrate
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Property | Enzyme | CA | pCA | BA | SA | AdoMet (CA) | AdoMet (pCA) | AdoMet (BA) | AdoMet (SA) |
| Km (μM) | CCMT1 | 124 (18) | 70.4 (3.0) | 45.5 (2.3) | 5.42 (0.78) | 1.14 (0.10) | 2.03 (0.09) | ||
| CCMT2 | 189 (33) | 77.0 (9.3) | 76.2 (6.2) | 13.3 (1.5) | 2.59 (0.19) | 2.60 (0.15) | |||
| CCMT3 | 196 (23) | 388 (42) | 17.3 (1.0) | 8.14 (0.43) | 3.96 (0.20) | 1.17 (0.10) | |||
| CCMT1-H160M | 577 (23) | 1200 (80) | 2430 (80) | 422 (35) | 5.70 (0.55) | 4.22 (0.43) | 11.1 (1.2) | 8.2 (0.9) | |
| Kcat | CCMT1 | 0.188 (0.007) | 0.084 (0.004) | 0.015 (0.001) | 0.198 (0.005) | 0.081 (0.003) | 0.018 (0) | ||
| CCMT2 | 0.251 (0.016) | 0.134 (0.003) | 0.020 (0) | 0.317 (0.015) | 0.106 (0.004) | 0.024 (0.001) | |||
| CCMT3 | 0.063 (0.001) | 0.050 (0.002) | 0.003 (0) | 0.090 (0.003) | 0.054 (0.003) | 0.004 (0) | |||
| CCMT1-H160M | 0.019 (0.001) | 0.009 (0.001) | 0.035 (0.002) | 0.003 (0) | 0.013 (0.001) | 0.008 (0.001) | 0.028 (0.002) | 0.006 (0) | |
| Kcat/Km | CCMT1 | 1,560 (165) | 1,190 (9) | 323 (6) | 37,700 (4500) | 72,100 (2900) | 8,720 (400) | ||
| CCMT2 | 1,390 (180) | 1,790 (210) | 266 (19) | 24,100 (1400) | 41,500 (1800) | 9,440 (700) | |||
| CCMT3 | 327 (33) | 132 (11) | 192 (8) | 11,100 (400) | 14,700 (500) | 3,590 (200) | |||
| CCMT1-H160M | 33.4 (1.5) | 7.21 (0.12) | 14.4 (0.6) | 7.19 (0.59) | 2,180 (230) | 2,020 (210) | 2,490 (110) | 690 (60) | |