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. 2007 Oct;19(10):3111–3126. doi: 10.1105/tpc.107.052621

Figure 5.

Figure 5.

Suppression of ACT1-Induced Dwarf Phenotype by Coexpression of Pollen-Specific PRF4.

(A) Approximately 7-week-old plants. A2P:A1, dwarf single transformant misexpressing ACT1; A2P:A1&P4, a double transformant misexpressing both ACT1 and PRF4 simultaneously. Note the suppression of dwarf phenotype in the double transgenic plant.

(B) Rosette leaves of just-bolted wild-type and single (A2P:A1) and double (A2P:A1&P4) transgenic plants.

(C) Protein gel blot analysis of ACT1 expression. Top panel shows a blot probed with MAb45a for ACT1. Both the dwarf single transformant misexpressing ACT1 and the normal double transgenic plant coexpressing ACT1 and PRF4 have same levels of ACT1 protein. The wild type has no detectable ACT1. Bottom panel shows a Coomassie blue–stained duplicate gel.

(D) Protein gel blot analysis of PRF4 expression. Top panel shows a blot probed with MAbPRF45 for PRF4 protein. Only the double transgenic plant shows strong PRF4 band. Bottom panel is a Coomassie blue–stained duplicate gel to show equal loading of total protein.