Figure 9.

Effect of Coexpression of Reproductive ACT1 and Constitutive ADF9 on Plant Development.

(A) Twenty-five-day-old wild-type plant, single transformants overexpressing ADF9 (A2P:ADF9) and misexpressing ACT1 (A2P:A1), and a double transformant (A2P:A1&ADF9) coexpressing ACT1 and ADF9. Note the dwarf stature of the double transformant.

(B) Protein gel blot analysis of ACT1 in leaf samples. Top blot probed with MAb45a to show ACT1 protein. Bottom panel probed with anti-PEPC antibody. Wild-type and the normal single transformant overexpressing ADF9 contain no ACT1 protein.

(C) RT-PCR analysis of ADF9 expression. The single A2P:ADF9 transformants and the double A2P:A1&ADF9 transgenic plants have >100 times the level of ADF9 transcripts than the wild type and plants misexpressing ACT1. Because of lack of ADF9-specific antibody, the levels of ADF9 expression are monitored here by RT-PCR analysis.

(D) Adult double transgenic plants coexpressing ACT1 and ADF7 (left) and ACT1 and ADF9 (right).

(E) Protein gel blot showing the levels of ACT1 protein in the double transgenic plants shown in (D). Probed with reproductive actin-specific MAb45a.