Figure 3.
Transgenic lymphomas and lymphoid hyperplasia are clonal. Southern blots were prepared with DNA from samples of lymphoma, hyperplasia, or normal spleens and hybridized to a PJ11 probe representing the JH region of the heavy chain locus. “Normal spleen” is a nontransgenic control spleen with the genomic 6.6-kb EcoRI fragment. The upper genomic band (23 kb) in mice of lineage 3 is caused by the integration of the transgene into the heavy chain locus in this lineage (lanes 1, 2, 3, 6, 9, and 10). Lanes 7, 8, 11, and 13 are from lineage 6. Lanes 4, 5, and 12 are from lineage 9.