Figure 4.

Expression of LMP1 correlates with IgH gene rearrangement and lymphoma detection. (A) Immunoblots were prepared with protein lysates from lymphoma samples and control tissues from three mice and the B95–8 cell line. Mouse A had lymphoma in hilar LN (lymph nodes) and the spleen, but not the skin and kidney. Mouse B had lymphoma in the mesenteric LN but not in the spleen. Mouse C had lymphoma in the lungs but not the spleen. “Normal spleen” was a spleen from a nontransgenic control mouse. (B) IgH gene rearrangement. Southern blots were hybridized to the PJ11 probe and showed the correlation of the lymphoma phenotype in the organs described in A with clonal rearrangement. In mouse A, gene rearrangement was analyzed and detected only in the spleen. All three mice were from lineage 3, which contained two genomic bands. The upper band (23 kb) resulted from the integration of the transgene into the heavy chain locus.