Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Feb 7;148(3):481–494. doi: 10.1083/jcb.148.3.481

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Dominant-active Cdc42 and Rac enhance calcium signaling, while dominant-negative Cdc42 and Rac dampen calcium signaling. The calcium responses of uninfected RBL-2H3 cells (a and d), cells infected with vaccinia virus expressing wild-type Cdc42 (b and e), wild-type Rac (c and f), Cdc42^V12 (g and k), Cdc42^N17 (h and l), Rac^V12 (i and m), and Rac^N17 (j and n), were compared with those of cells containing empty vector. Cells were infected for 6 h at 37°C and loaded with Indo-1. Overall calcium responses were measured in a fluorescence spectrophotometer in response to stimulation with 100 ng/ml DNP/BSA (a–c and g–j, unfilled arrows). To specifically measure the release of calcium from ER stores (d–f and k–n), loaded cells were treated with 4 mM EDTA (filled arrows) and then stimulated with 100 ng/ml DNP/BSA (unfilled arrows). For each form of Cdc42 and Rac, measurements of the calcium responses in the absence and presence of EGTA were performed concurrently. Traces shown are representative of three independent experiments.

Dominant-active Cdc42 and Rac enhance calcium signaling, while dominant-negative Cdc42 and Rac dampen calcium signaling. The calcium responses of uninfected RBL-2H3 cells (a and d), cells infected with vaccinia virus expressing wild-type Cdc42 (b and e), wild-type Rac (c and f), Cdc42^V12 (g and k), Cdc42^N17 (h and l), Rac^V12 (i and m), and Rac^N17 (j and n), were compared with those of cells containing empty vector. Cells were infected for 6 h at 37°C and loaded with Indo-1. Overall calcium responses were measured in a fluorescence spectrophotometer in response to stimulation with 100 ng/ml DNP/BSA (a–c and g–j, unfilled arrows). To specifically measure the release of calcium from ER stores (d–f and k–n), loaded cells were treated with 4 mM EDTA (filled arrows) and then stimulated with 100 ng/ml DNP/BSA (unfilled arrows). For each form of Cdc42 and Rac, measurements of the calcium responses in the absence and presence of EGTA were performed concurrently. Traces shown are representative of three independent experiments.