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. 2000 Feb 7;148(3):481–494. doi: 10.1083/jcb.148.3.481

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Activated Cdc42 interacts with PLCγ1 in vitro. Lysates from (a) unstimulated RBL-2H3 cells and (b) cells stimulated with 100 ng/ml DNP/BSA for 5 min were incubated with immobilized GST (lane 2), GST-Cdc42 (lane 3), GST-Cdc42^L61 (lane 4), and GST-Cc42^N17 (lane 5) for 1 h at 4°C. Lane 1 contains whole cell lysate used in the binding reactions. Bound proteins were washed, resolved by SDS-PAGE, and analyzed by Western blots using either anti-PLCγ1 antibody (upper panel) or anti-IQGAP antibody (bottom panel).