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. 2000 Feb 7;148(3):481–494. doi: 10.1083/jcb.148.3.481

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Cells infected with the effector domain mutant Cdc42^V12,Y40 exhibit impaired calcium signaling compared with cells infected with Cdc42^V12. (a) Lysates from RBL-2H3 cells infected with vaccinia virus containing empty vector (lane 1), or a vector expressing Cdc42 (lane 2), Cdc42^V12 (lane 3), Cdc42^N17 (lane 4), and Cdc42^V12,Y40 (lane 5) were treated as described in the legend to Fig. 1, a and b. (b) The calcium responses of RBL-2H3 cells infected with Cdc42^V12,Y40 were compared with those of Cdc42^V12 and empty vector upon antigen stimulation (unfilled arrows) in the absence or presence (c) of 4 mM EGTA (filled arrow). Traces shown are representative of three independent experiments.