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. 2000 Feb 7;148(3):519–530. doi: 10.1083/jcb.148.3.519

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Comet tail formation in vivo induced by fertilization and PMA treatment. (A) Gallery of confocal images showing actin comet tails in eggs fixed 8 min after fertilization. F-actin was visualized by fluorescein-phalloidin staining. (B) In vivo time-lapse sequence (16-s intervals) showing rhodamine-actin comet tails (indicated by arrowheads) 20 min after treatment with 2 μM PMA. See Online Supplemental Material, Video 2 (http://www.jcb.org/cgi/content/full/148/3/519/DC1). Bars, 10 μm.