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. 2000 Feb 7;148(3):519–530. doi: 10.1083/jcb.148.3.519

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Gallery of electron micrographs depicting N-WASP immunogold-labeled HeLa vesicles associated with actin comet tails. Cell-free motility reactions containing unlabeled HeLa membranes were fixed in perfusion chambers, immunolabeled with affinity-purified N-WASP antibodies followed by 15 nm protein A–gold, and processed for electron microscopy. Gold particles are occasionally visible in the comet tails, but the vast majority are associated with the membrane. Note the similarity in vesicle morphology to the Xenopus egg vesicles shown in Fig. 6. Bar, 200 nm.