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. 2000 May 29;149(5):1011–1018. doi: 10.1083/jcb.149.5.1011

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© 2000 The Rockefeller University Press

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graphic file with name JCB0003107.f1ah.jpg — BAX and cytochrome c undergo subcellular redistribution during trophic factor withdrawal in immature, but not mature, sympathetic neurons. Neurons were maintained in NGF for ∼5 (immature; DIV 5) or 25 d (mature; DIV 25) and then deprived of NGF in the presence of 50 μM BAF. At the indicated times, neurons were fixed and immunostained for BAX (a–d, i) or cytochrome c (j). All cells were costained with Hoechst 33258 (e–j). The number of cells exhibiting punctate BAX staining or diffuse cytochrome c staining was determined, with 200–250 cells counted for each condition. Mean ± SEM, n ≥ 3. Bar, 5 μm. Please note that a, b, e, and f have been reported previously in Putcha et al. 1999 and are included only for comparison.

graphic file with name JCB0003107.f1ij.jpg — BAX and cytochrome c undergo subcellular redistribution during trophic factor withdrawal in immature, but not mature, sympathetic neurons. Neurons were maintained in NGF for ∼5 (immature; DIV 5) or 25 d (mature; DIV 25) and then deprived of NGF in the presence of 50 μM BAF. At the indicated times, neurons were fixed and immunostained for BAX (a–d, i) or cytochrome c (j). All cells were costained with Hoechst 33258 (e–j). The number of cells exhibiting punctate BAX staining or diffuse cytochrome c staining was determined, with 200–250 cells counted for each condition. Mean ± SEM, n ≥ 3. Bar, 5 μm. Please note that a, b, e, and f have been reported previously in Putcha et al. 1999 and are included only for comparison.