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. 2000 May 1;149(3):657–666. doi: 10.1083/jcb.149.3.657

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Inhibition of NFAT activity by the expression of GFP-VIVIT does not inhibit myogenesis in L6 muscle cell cultures. (a) Primary myoblasts were infected with an NFAT responsive reporter and either control (Ctrl) or GFP-VIVIT retroviruses. Cells were induced to differentiate for 48 h in DM. Luciferase assays were performed on vehicle (V), ionomycin (I, 1 μM) plus PMA- (P, 10 nM), or IP plus CSA- (C, 1 μM) treated cultures. GFP-VIVIT inhibits NFAT activity in skeletal muscle cells. Data are reported as the fold increase over vehicle-treated control cells. Each bar represents the mean ± SEM of three independent experiments each performed in triplicate. (b) L6 myoblasts were infected with either control or GFP-VIVIT retroviruses and induced to differentiate in DM. Cellular proteins were collected at various time points and analyzed by immunoblotting. GFP-VIVIT does not inhibit the expression of either myogenic marker. The blot shown is representative of two independent experiments.

Inhibition of NFAT activity by the expression of GFP-VIVIT does not inhibit myogenesis in L6 muscle cell cultures. (a) Primary myoblasts were infected with an NFAT responsive reporter and either control (Ctrl) or GFP-VIVIT retroviruses. Cells were induced to differentiate for 48 h in DM. Luciferase assays were performed on vehicle (V), ionomycin (I, 1 μM) plus PMA- (P, 10 nM), or IP plus CSA- (C, 1 μM) treated cultures. GFP-VIVIT inhibits NFAT activity in skeletal muscle cells. Data are reported as the fold increase over vehicle-treated control cells. Each bar represents the mean ± SEM of three independent experiments each performed in triplicate. (b) L6 myoblasts were infected with either control or GFP-VIVIT retroviruses and induced to differentiate in DM. Cellular proteins were collected at various time points and analyzed by immunoblotting. GFP-VIVIT does not inhibit the expression of either myogenic marker. The blot shown is representative of two independent experiments.