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. 2000 May 1;149(3):603–612. doi: 10.1083/jcb.149.3.603

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Kinetics of golgin-160 cleavage during apoptosis. (a) HeLa lysates were made at 1, 2, 4, and 6 h after addition of 1 μm staurosporine, and were run on a 7.5% (top) or 10% (bottom) SDS-PAGE. 70 μg of lysate was loaded in each lane. Golgin-160 and PARP were detected by immunoblotting with anti–golgin-160(648) or patient serum recognizing PARP, respectively. Fragments are marked on the right. (b) Graph showing a decrease of the p163 fragment concomitant with an increase in the p140 fragment. Percentage of maximum fragment plotted against time in staurosporine demonstrates that the caspase-2–generated p163 band reaches a maximum at 2 h, whereas the Group II caspase-generated p140 band reaches its maximum at 4 h in staurosporine. The kinetics of the formation of the p140 fragment of golgin-160 is very similar to that for the p89 fragment of PARP.