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. 2000 May 1;149(3):603–612. doi: 10.1083/jcb.149.3.603

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The NH₂-terminal fragment of golgin-160 is released from Golgi membranes after cleavage by caspase-2. Untreated HeLa cells (a–d) and HeLa cells treated with 1 μm staurosporine for 1 h (e–h) were fixed, permeabilized, and triple labeled with anti–golgin-160(N) (a and e), which was visualized as red; antigiantin (b and f), which was visualized as green; and DAPI (c and g), which was visualized as blue. Overlapping red and green pixels appear yellow (d and h). Arrow in h denotes an apoptotic cell, which no longer stains for the NH₂-terminal portion of golgin-160. These results are representative of three separate experiments. Bars, 10 μm.