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. 2000 May 1;149(3):667–682. doi: 10.1083/jcb.149.3.667

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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TGF-β receptor expression during adipocyte differentiation of 3T3-F442A cells. (A) Northern analysis of TβRII and TβRI mRNA expression on 2 μg of poly(A)⁺ RNA from preadipocytes (pread) or 8-d after confluent adipocytes (ad). Levels of C/EBPβ mRNA are shown as a loading control. (B) Cell-surface availability of TGF-β receptors, as assessed by chemical cross-linking to [¹²⁵I]TGF-β, decreases during differentiation. TGF-β binding was assessed in confluent preadipocytes (pread) and at 1, 3, and 10 d after confluence. The approximate positions of type III (RIII), type II (RII), and type I (RI) receptors are indicated.