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. 2000 May 1;149(3):667–682. doi: 10.1083/jcb.149.3.667

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Effect of Smad2, Smad3, or their dominant negative mutants on adipogenesis. (A) Expression of Flag-tagged Smads in their respective stable cell lines. Whole cell lysates were immunoprecipitated using anti-Flag–conjugated beads, followed by Western blotting with anti-Flag antibody. Position of background IgG is indicated. (B and C) The effect of overexpression of Smad2, Smad3, or their dominant negative mutants on lipid accumulation in adipogenic differentiation. Untreated cells (B) and cells treated with 1 ng/ml (C) at 8 d after confluence are shown.