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. 2000 May 1;149(3):667–682. doi: 10.1083/jcb.149.3.667

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Generation and differentiation of Smad6 or Smad7 overexpressing cell lines. (A) Whole cell lysates of vector control, Smad6, or Smad7 expressing cells were analyzed by anti-Flag immunoprecipitation and anti-Flag Western blotting. (B) Lipid accumulation in Smad6 and Smad7 overexpressing cell lines. Vector control and Smad6 or Smad7-overexpressing cell lines were grown under differentiation conditions in the absence or presence of 1 ng/ml TGF-β. At 8 d after confluence, the cells were fixed and stained with Oil Red O.