Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 May 1;149(3):647–656. doi: 10.1083/jcb.149.3.647

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Localization of the Na,K ATPase in β spectrin mutants by confocal microscopy. Dissected preparations of the middle midgut from wild-type control larvae (A and B) and β-spec^em6 mutants (C and D) were stained with mouse monoclonal anti–Na,K ATPase α subunit and fluorescent secondary antibody. The basolateral staining pattern of the Na,K ATPase in wild-type appeared as rings in en face views (A), or as horseshoe shapes in optical sections near the center of the midgut (B). Neither pattern was detectable in the β-spec^em6 mutants (C and D). Toward the anterior (C and D, arrowhead), Na,K ATPase staining was distributed throughout the cytoplasm. Toward the posterior (C and D, arrow), staining appeared as large puncta and did not appear to be associated with the plasma membrane. In between these two regions, staining was relatively weak or in some cases absent. Bar, 10 μM.