Figure 5.

LSD1, EDS1, and PAD4 Control Aerenchyma Formation in Arabidopsis.

(A) Toluidine blue–stained cross sections of Arabidopsis Ws-0, lsd1, lsd1 eds1-1, and lsd1 pad4-5 hypocotyls of plants subjected to waterlogging for 6, 7, and 8 d. Representative samples are shown (n = 8). Arrows indicate parenchyma (6 d) and aerenchymatous lacunae (7 and 8 d).

(B) Toluidine blue–stained cross sections of Arabidopsis eds1-1 and pad4-5 hypocotyls waterlogged for 7 d. Representative samples are shown (n = 8). White arrows indicate parenchyma (6 d waterlogged) and aerenchymatous lacunae (7 d).

(C) Quantification of cross-section areas of aerenchymatous lacunae in Arabidopsis Ws-0, lsd1, eds1-1 lsd1, pad4-5 lsd1, eds1-1, and pad4-5 hypocotyls waterlogged for 7 d (*P < 0.05, Students t test, n = 8, error bars are ± se). The numbers beneath each column denote the percentage of plants that formed aerenchyma.