Figure 1.

sad2-1 Is a UV-B–Tolerant Mutant.

(A) and (B) Twelve-day-old wild-type (left panel) and sad2-1 ([A]; right panel) mutant or sad2-2 ([B]; right panel) seedlings were treated with UV-B (5.3 mW/cm²) for 10 and 12 min, respectively. After treatment, plants were transferred to a continuous light growth chamber for 3 d and then photographed.

(C) Expression of SAD2 in sad2-1 and sad2-2. Total RNA was extracted from 12-d-old seedlings of wild-type and the sad2 mutants. The resulting cDNAs were used for real-time PCR analysis. Error bars indicate sd (n = 3).