Figure 3.

sad2-1 Accumulates More UV-Absorbing Pigments.

(A) Absorption spectra of pigments in the wild type (thin line) and the sad2-1 mutant (bold line) under normal growth conditions.

(B) Content of UV-absorbing pigments at an absorption spectrum value of 330 nm. Pigments were extracted from 12-d-old seedlings with or without UV-B (0.04 mW/cm²) treatment. Vertical bars represent se for three independent experiments.

(C) LC-MS identification of soluble phenolic compounds from sad2-1 and the wild type. Fourteen-day-old wild-type C24 and sad2-1 seedlings with or without UV-B treatment were used for methanol:water (4:1) extraction and liquid chromatography. IS, internal standard; the main flavonol glycosides: K, kaempferol; Q, quercetin; I, isorhamnetin; the main sinapoyl derivatives: E and Z isomers of SG, E and Z isomers of SM.