Table 1.
LC-MS Determination of Soluble Phenolics
| Compounds | (M-H) | C24 | C24+UV | sad2-1 | sad2-1+UV |
|---|---|---|---|---|---|
| ng/150 mg Fresh Plant Materials | |||||
| Flavonol glycosides | |||||
| K1 | 577 | 26 (4) | 44 (4) | 59 (2) | 91 (19) |
| K2 | 593 | 47 (3) | 84 (6) | 97 (5) | 144 (35) |
| K3 | 739 | 31 (1) | 62 (5) | 71 (2) | 123 (33) |
| K4 | 755 | 50 (4) | 86 (7) | 121 (6) | 185 (47) |
| Q1 | 609 | 57 (4) | 122 (3) | 117 (1) | 207 (47) |
| Q2 | 755 | 8 (1) | 21 (3) | 14 (2) | 27 (6) |
| Q3 | 771 | 22 (5) | 35 (5) | 28 (2) | 44 (6) |
| I1 | 623 | 9 (1) | 20 (2) | 23 (1) | 46 (9) |
| Sinapoyl derivatives | |||||
| SM | 339 | 10 (3) | 17 (2) | 15 (3) | 19 (1) |
| SG | 385 | 18 (2) | 29 (9) | 58 (1) | 85 (22) |
Flavonol glycosides: K, kaempferol; Q, quercetin; I, isorhamnetin. K1, kaempferol 3-O-rhamnoside 7-O-rhamnoside; K2, kaempferol 3-O-glucoside 7-O-rhamnoside; K3, kaempferol 3-O-[6″-O-(rhamnosyl) glucoside] 7-O-rhamnoside; K4, kaempferol 3-O-[6″-O-(glucosyl) glucoside] 7-O-rhamnoside. Q1, quercetin 3-O-glucoside 7-O-rhamnoside; Q2, quercetin 3-O-[6″-O-(rhamnosyl) glucoside] 7-O-rhamnoside; Q3, quercetin 3-O-[6″-O-(glucosyl) glucoside] 7-O-rhamnoside. I1, isorhamnetin 3-O-glucoside 7-O-rhamnoside. Sinapoyl derivatives: E and Z isomers of SG, E and Z isomers of SM. Values represent means (se in parentheses) from three independent experiments with 40 plants each. LC-MS quantitative determination was as described by Abdulrazzak et al. (2006).