Fast-Elongating Cotton Fibers Contain Significantly Higher Levels of VLCFAs but Not LCFAs.
(A) GC analyses of LCFAs and VLCFAs from 7-DPA fiber cells (top panel), 7-DPA wild-type ovules with fibers stripped off (middle panel), and 1-DPA wild-type ovules (bottom panel).
(B) GC analysis of LCFAs and VLCFAs from 7-DPA (top panel) and 1-DPA (bottom panel) fl mutant ovules.
(C) GC analysis of LCFAs and VLCFAs from wild-type cotton ovules treated with 2 μM ACE for 6 d (top panel) or 1 d (bottom panel).
Similar results were obtained from three independent ovule cultures, with quantitative analysis reported in Supplemental Table 3 online. In general, ∼55% of the original C17:0 was recovered after the extraction processes in this and all other related experiments. See Figure 2 legend for different scaling of the chromatograms.