Figure 2.

Colchicine-Induced Polyploidization Partly Rescues the Cell and Organ Size Phenotype in bin4.

(A) Flow cytometric analysis of 12-d-old, light-grown seedlings.

(B) FISH analysis of endoreduplicated polytene nuclei in wild-type diploid plants and polyploid nuclei in colchicine-induced tetraploid plants.

(C) Scanning electron micrographs of 14-d-old leaf epidermis.

(D) Twelve-day-old, light-grown seedlings and mature flowers. Organ size is more than doubled in bin4 tetraploid plants. Polyploidization appears to have much less impact on the wild-type organ size, but its flower size is significantly increased.

Col and Col-0, Columbia ecotype; DAPI, 4′,6-diamidino-2-phenylindole. Bars = 10 μm in (B), 200 μm in (C), 1 mm in (D) (seedlings), and 5 mm in (D) (flowers).