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Coimmunoprecipitation of RBP40 and Nac2.
Stromal protein fractions from wild-type and nac2 chloroplasts were incubated with either αRBP40 antibody coupled to protein A–Sepharose (IP αRBP40) (A) or αNac2 antibody coupled to protein G–Sepharose (IP αNac2) (B). After elution from the matrix, the material was subjected to SDS-PAGE and immunolabeled using the same antibodies (ID αNac2 or ID αRBP40). The asterisk marks material that cross-reacts with the αNac2 antiserum.
